Protein computation, or the determination of protein content, involves various methods to quantify the amount of protein present in a food, substance, or biological sample. For example, the Kjeldahl method measures nitrogen content, which is then converted to an estimated protein value using a conversion factor. Other approaches, such as the Dumas method and various spectrophotometric assays (like the Biuret and Bradford assays), provide alternative means of quantifying protein.
Accurate protein quantification is essential in diverse fields. In nutrition, it informs dietary choices and helps manage health conditions. In research, it enables precise experimentation and analysis of biological processes. Historically, the need to understand protein content stems from recognizing its fundamental role in growth, development, and overall health. Accurate measurement techniques have evolved significantly over time, supporting advancements in food science, medicine, and agriculture.